The Journal of Vitaminology 13, 283-288 (1967) the Effects of Dietary Amino Acid and pro - Tein Compositions on Coa Metabolism in Young Albino Rats1

1. Assay Method of CoA CoA assay method was based on Kaplan-Lipmann's method (2). The hepatic nitrogen content was determined by micro-Kjeldahl's method. Male albino rats of Wistar strain weighing 50 g were fed on a commercial solid feed for the first day and on a 18 % milk casein feed for the next two days. The animals were then divided into two groups and fed on a synthetic diet as shown in Table 1. The hepatic weight, nitrogen content and CoA content were determined 1, 2 and 4 weeks later. For studies on the influence of dietary amino acid composition, the animals were placed on a preliminary feeding period as mentioned above. They were divided into seven groups from A to G and were fed on a sythetic diet correspond ing to 1/8 of the body weight as shown in Table 2 for ten days prior to the ex periment. The control group (C) received 5 % milk casein enriched with 9 essential amino acids suggested to be essential for the growth of albino rats by Rose (3),